DNA Sequencing Protocols

DNA Sequencing Protocols

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DNA Sequencing Protocols provides you with the knowledge to become a sequencing expert. An all-star cast of investigators covers virtually all aspects, including developments in cycle sequencing, sequencing PCR products, sequencing lambda and cosmids, multiplex sequencing, direct blotting electrophoresis, sequencing of chemiluminescence, and automated sequencing.Col 7: T7 DNA polymerase (5 U/uL), 6 I¼L/cycle. 6. Col 8: ddA ... series of sequencing reactions should include one control reaction using high quality DNA . ... In the following troubleshooting guide it is assumed that the control reaction works.

Title:DNA Sequencing Protocols
Author: Hugh G. Griffin
Publisher:Springer Science & Business Media - 1993

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